Evaluation of DNA damage and repair in Radiographers and Dental Surgeons using X-ray machines in Dental Clinics.
DOI:
https://doi.org/10.22399/ijnasen.14Keywords:
DNA damage, DNA repair, Radiographers, Dental SurgeonsAbstract
Acute exposure to high dose of X-rays has potential to damage the DNA and chronic exposure to low dose radiation can also be deleterious because of the cumulative effects of X-ray. DNA damage is an indicator for various health problems including reproductive failure and cancer. Low dose X-ray radiation is used in medical diagnostics and dental clinics, occupational exposure to this may have harmful effects in the absence of necessary precautions. Some studies from other part of the world and a few from India have been carried out to identify the adverse effects of X-rays used for diagnostic purpose. However, the results from these studies are not consistent. Therefore, it is planned to study DNA damage in Dental Surgeons and Radiographers from Hyderabad, who use X-rays routinely in their clinics. Two sensitive techniques, Comet assay and Micronucleus test will be employed for evaluation. This study would help in providing guidelines for better health of Dental Surgeons and Radiographers
References
[1]Ameerunnisa, Chaya M, David G, Savitha BK, Ramnarayan and Sanjay CJ. (2011). Analysis of cytogenetic effects of radiation in dental personnel exposed to diagnostic X-rays. Int J Hum Genet, 11(4): 271-276.
[2]Maluf SW, Passos DF, Bacelar A, Speit G, Erdtmann B. (2001). Assessment of DNA damage in lymphocytes of workers exposed to X-radiation using the micronucleus test and the comet assay. Environ Mol Mutage, 38(4): 311–315.
[3]Miyaji CK, Cólus LM. (2002). Cytogenetic biomonitoring of brazilian dentists occupationally exposed to low doses of X-radiation. Pesqui Odontol Bras, 16(3): 196-201.
[4]Maddileti U, Padmaja T, Hema Prasad and Reddy PP. (2002). Analysis of chromosomal aberrations in the peripheral lymphocytes of workers exposed to diagnostic X-rays. Int J Hum Genet, 2(4):265-268.
[5] Ahuja YR and Saran R. (1999). Alkaline single cell gel electrophoresis assay I. Protocol. J.Cytol. Gene. 34: 57-62.
[6]Goud KI, Hasan Q, Balakrishna N, Rao KP, Ahuja YR. (2004). Genotoxicity evaluation of individuals working with photocopying machines. Mutat Res, 563(2): 151-158.
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